The ACRF Cancer Genomics Facility has expertise in a wide range of Sequencing, Microarray and related applications. Please contact the Facility (Rob King, 8222 3966, Rob.King@sa.gov.au) for more information or to enquire about any applications not listed below.
|
Service |
Sample type |
|---|---|
|
Sanger Sequencing |
|
|
Amplicon DNA, Plasmid DNA |
DNA, primers |
|
NGS - DNA sequencing |
|
|
Whole genome sequencing |
Genomic DNA |
|
Whole exome sequencing |
Genomic DNA |
|
Targeted DNA sequencing (pre-designed gene panels, germline and somatic) |
Genomic DNA |
|
TruSight Oncology 500 panel sequencing |
FFPE tissue DNA and RNA |
|
Targeted DNA sequencing (custom) |
Genomic DNA |
|
ChIP-seq, ATAC-seq |
Prepared DNA |
|
Long read genome sequencing (Oxford Nanopore, PacBio) |
Genomic DNA |
|
Long read amplicon sequencing (Oxford Nanopore, PacBio) |
Amplicons |
|
NGS - RNA sequencing |
|
|
mRNA sequencing (polyA+) |
Total RNA |
|
Whole transcriptome sequencing (rRNA/globin depleted) |
Total RNA |
|
Small RNA (eg miRNA) sequencing |
Total RNA |
|
Targeted RNA sequencing (custom) |
Total RNA |
|
CLIP-seq |
Prepared RNA or prepared cDNA |
|
NGS - Single Cell Sequencing |
|
|
Single Cell Gene Expression (10X Genomics) |
Prepared cells |
|
Single Cell Immune Profiling (10X Genomics) |
Prepared cells |
|
Feature Barcoding/CITE-seq (10X/Biolegend TotalSeq applications) |
Prepared cells |
|
Single Cell ATAC-seq (10X Genomics) |
Prepared cells |
|
NGS – Spatial Transcriptomics |
|
|
10X Genomics Visium |
Tissue |
|
NGS - Sequencing only |
|
|
Sequencing of client-prepared libraries |
Libraries (Illumina or PacBio) |
|
Microarray services |
|
|
Illumina BeadChip (eg Global Screening Array, CytoSNP arrays, MethylationEPIC) |
Genomic DNA |
|
GeneChip (Affymetrix/ThermoFisher) Gene Expression Arrays (cartridge format) |
Total RNA |
|
qPCR |
|
|
Fluidigm Biomark qPCR analysis |
Total RNA |
|
Custom Library Preparation |
|
|
Please enquire |
|
|
Sample QC |
|
|
DNA or RNA analysis (PerkinElmer LabChip GX, Agilent Bioanalyzer) |
DNA, RNA |
|
Genomic DNA analysis (Agilent FemtoPulse) |
Genomic DNA |
|
Long read library analysis (Agilent FemtoPulse) |
Library DNA |
