The ACRF Cancer Genomics Facility has expertise in a wide range of Sequencing, Microarray and related applications. Please contact the Facility (Rob King, 8222 3966, Rob.King@sa.gov.au) for more information or to enquire about any applications not listed below.
| Service | Sample type |
| Sanger Sequencing | |
| Amplicon DNA, Plasmid DNA | DNA, primers |
| NGS - DNA sequencing | |
| Whole genome sequencing | Genomic DNA |
| Whole exome sequencing | Genomic DNA |
| Targeted DNA sequencing (pre-designed gene panels, germline and somatic) | Genomic DNA |
| TruSight Oncology 500 panel sequencing | FFPE tissue DNA and RNA |
| Targeted DNA sequencing (custom) | Genomic DNA |
| ChIP-seq, ATAC-seq | Prepared DNA |
| Long read genome sequencing (Oxford Nanopore, PacBio) | Genomic DNA |
| Long read amplicon sequencing (Oxford Nanopore, PacBio) | Amplicons |
| NGS - RNA sequencing | |
| mRNA sequencing (polyA+) | Total RNA |
| Whole transcriptome sequencing (rRNA/globin depleted) | Total RNA |
| Small RNA (eg miRNA) sequencing | Total RNA |
| Targeted RNA sequencing (custom) | Total RNA |
| CLIP-seq | Prepared RNA or prepared cDNA |
| NGS - Single Cell Sequencing | |
| Single Cell Gene Expression (10X Genomics) | Prepared cells |
| Single Cell Immune Profiling (10X Genomics) | Prepared cells |
| Feature Barcoding/CITE-seq (10X/Biolegend TotalSeq applications) | Prepared cells |
| Single Cell ATAC-seq (10X Genomics) | Prepared cells |
| NGS – Spatial Transcriptomics | |
| 10X Genomics Visium | Tissue |
| NGS - Sequencing only | |
| Sequencing of client-prepared libraries | Libraries (Illumina or PacBio) |
| Microarray services | |
| Illumina BeadChip (eg Global Screening Array, CytoSNP arrays, MethylationEPIC) | Genomic DNA |
| GeneChip (Affymetrix/ThermoFisher) Gene Expression Arrays (cartridge format) | Total RNA |
| qPCR | |
| Fluidigm Biomark qPCR analysis | Total RNA |
| Custom Library Preparation | |
| Please enquire | |
| Sample QC | |
| DNA or RNA analysis (PerkinElmer LabChip GX, Agilent Bioanalyzer) | DNA, RNA |
| Genomic DNA analysis (Agilent FemtoPulse) | Genomic DNA |
| Long read library analysis (Agilent FemtoPulse) | Library DNA |
