Selected Publications prior to 2007

2006

Fotia AB, Cook DI, Kumar S (2006) The ubiquitin-protein ligases Nedd4 and Nedd4-2 show similar ubiquitin-conjugating enzyme specificities. Int. J. Biochem. Cell Biol. 23: 472-479.

Rauh R, Dinudom A, Fotia AB, Paulides M, Kumar S, Korbmacher C, Cook DI (2006) Stimulation of the epithelial sodium channel (ENaC) by the serum- and glucocorticoid-inducible kinase (Sgk) involves the PY-motifs of the channel but is independent of sodium feedback inhibition. Pflug. Arch. Eur. J. Physiol. 452: 290–299.

Shearwin-Whyatt L, Dalton H, Foot N, Kumar S (2006) Regulation of functional diversity within the Nedd4 family by accessory and adaptor proteins. BioEssays 28: 617-628.

Dorstyn L, Kumar S (2006) A cytochrome c-free fly apoptosome. Cell Death Differ. 13: 1049- 1051.

Mills K, Daish, T, Harvey KF, Pfleger CM, Hariharan IK, Kumar S (2006) The Drosophila melanogaster Apaf-1 homologue ARK is required for most, but not all, programmed cell death. J. Cell Biol. 172: 809-815.

Sang Q, Kim MH, Kumar S, Bye N, Morganti-Kossman MC, Gunnersen J, Fuller S, Howitt J, Hyde L, Beissbarth T, Scott HS, Silke J, Tan SS (2006) Nedd4-WW domain-binding protein 5 (Ndfip1) is associated with neuronal survival after acute cortical brain injury. J. Neurosci. 26: 7234-7244.

Daish T, Kumar S (2006) Biology of caspases. In: Apoptosis, Cell Signaling and Human Diseases: Molecular Mechanisms Vol 2 (R. Srivastava, Editor). The Humana Press, Inc. pp 347-363.

2005

Mills K, Daish T, Kumar S (2005) The function of the Drosophila caspase DRONC in cell death and development. Cell Cycle 4:744-746.

Kilpatrick ZE, Cakouros D, Kumar S (2005) Ecdysone-mediated upregulation of the effector caspase DRICE is required for hormone-dependent apoptosis in Drosophila cells. J. Biol. Chem. 280: 11981- 11986.

2004

Daish T, Cakouros D, Kumar S (2003) Distinct promoter regions regulate spatial and temporal expression of the Drosophila caspase dronc. Cell Death Differ. 10: 1348-1356.

Cakouros D, Daish TJ, Mills K, Kumar S (2004) An arginine-histone methyl transferase, CARMER, coordinates ecdysone-mediated apoptosis in Drosophila cells. J. Biol. Chem. 279:18467-18471.

Cakouros D, Daish TJ, Kumar S (2004) Ecdysone receptor directly binds the promoter of the Drosophila caspase dronc regulating its expression in specific tissues. J. Cell Biol. 165: 631- 640.

Ekert PG, Read SH, Silke J, Marsden V, Kaufmann H, Hawkins CJ, Gerl R, Kumar S, Vaux DL (2004) Apaf-1, caspase-2 and caspase-9 accelerate apoptosis, but do not determine whether factor-deprived or drug-treated cells die. J. Cell Biol. 165: 835-842.

Murdaca J, Treins C, Monthouël-Kartmann MN, Pontier-Bres R, Kumar S, Van Obberghen E, Giorgetti-Peraldi S (2004) Grb10 prevents Nedd4-mediated VEGF receptor-2 degradation. J. Biol. Chem. 279: 26754-26761.

Fotia AB, Ekberg J, Cook DI, Adams DJ, Poronnik P, Kumar S (2004) Regulation of neuronal voltage-gated sodium channels by the ubiquitin-protein ligases Nedd4 and Nedd4-2. J. Biol. Chem. 279: 28930-28935.

Kumar S, Cakouros D (2004) Transcriptional control of the core cell death machinery. Trends Biochem. Sci. 29: 193-199.

Kumar S (2004) Migrate, differentiate, proliferate or die: Pleiotropic functions of an apical “apoptotic caspase”. Science STKE 2004: pe49.

Shearwin-Whyatt LM, Brown DL, Wylie FG, Stow JL, Kumar S (2004) N4WBP5A (Ndfip2), a Nedd4-interacting protein, localizes to multivesicular bodies and the Golgi, and has a potential role in protein trafficking. J. Cell Sci. 117: 3679-3689.

Dinudom A, Fotia A, Lefkowitz RJ, Young JA, Kumar S, Cook DI (2004) The kinase Grk2 regulates the Nedd4/Nedd4-2 dependent control of epithelial Na+ channels. Proc. Natl. Acad. Sci. USA. 101: 11886-11890.

Baliga BC, Read SH, Kumar S (2004) The biochemical mechanism of caspase-2 activation. Cell Death Differ. 11: 1234-1241.

Dorstyn L, Mills K, Lazebnik Y, Kumar S (2004) The two cytochrome c species, DC3 and DC4, are not required for caspase activation and apoptosis in Drosophila cells. J. Cell Biol. 167: 405-410.

Chew SK, Akdemir F, Chen P, Lu, W-J, Mills K, Daish, T, Kumar S, Rodriguez A, Abrams JM (2004) The apical caspase, dronc, governs programmed and unprogrammed cell death in Drosophila. Developmental Cell 7: 897-907.

Daish TJ, Mills K, Kumar S (2004) Drosophila caspase DRONC is required for specific developmental cell death pathways and stress-induced apoptosis. Developmental Cell 7: 909-915.

Hryciw DH, Ekberg J, Lee A, Lensink IL, Kumar S, Guggino WB, Cook DI, Carol A. Pollock CA, Poronnik P (2004) Nedd4-2 functionally interacts with ClC-5: involvement in constitutive albumin endocytosis in proximal tubule cells. J. Biol. Chem. 279: 54996-55007.

Dorstyn L, Kumar S (2004) Programmed cell death in Drosophila melanogaster. In: When Cells Die II- A comprehensive evaluation of apoptosis and programmed cell death (R. A. Lockshin and Z. Zakeri eds.), John Wiley & Sons, Inc., New York, pp 79-97.

Kumar S (2004) Measurement of caspase activity in cells undergoing apoptosis. In: Apoptosis Methods and Protocols (H.J.M. Brady ed.), Methods in Molecular Biology 282:19-30.

2003

Baliga C, Kumar S (2003) The Apaf-1/cytochrome c apoptosome: an essential initiator of caspase activation or just a sideshow? Cell Death Differ. 10: 15-17.

Fotia A, Dinudom A, Shearwin KE, Koch J-P, Korbmacher C, Cook DI, Kumar S (2003) The role of individual Nedd4-2 (KIAA0439) WW domains in binding and regulating epithelial sodium channels. FASEB J. 17: 70-72.

Baliga BC, Colussi PA, Read SH, Dias MM, Jans DA, Kumar S (2003) Role of prodomain in importin-mediated nuclear localization and activation of caspase-2. J. Biol. Chem. 278: 4899-4905.

Kim TW, Hung C-F, Ling M, Juang J, He L, Hardwick JM, Kumar S, Wu T-C (2003) Enhancing DNA vaccine potency by coadministration of DNA encoding antiapoptotic proteins. J. Clin. Invest. 112: 110-117.

Quinn L, Coomb M, Mills K, Daish T, Colussi P, Kumar S, Richardson H (2003) Buffy, a Drosophila Bcl-2 related protein, has anti-apoptotic and cell cycle inhibitory function. EMBO J. 22: 3568-3579.

Daish T, Cakouros D, Kumar S (2003) Distinct promoter regions regulate spatial and temporal expression of the Drosophila caspase dronc. Cell Death Differ. 10: 1348-1356.

2002

Shcherbik N, Kumar S, Haines DS (2002) Substrate proteolysis is inhibited by dominant-negative Nedd4 and Rsp5 mutants harboring alterations in WW domain 1. J. Cell Sci. 115: 1041-1048.

Harvey KF, Shearwin-Whyatt LM, Fotia A, Parton RG, Kumar S (2002) N4WBP5, a potential target for ubiquitination by the Nedd4 family of proteins, is a novel Golgi-associated protein. J. Biol. Chem. 277: 9307-9317.

Dorstyn L, Read S, Cakouros D, Huh JR, Hay BA, Kumar S (2002) The role of cytochrome c in caspase activation in Drosophila cells. J. Cell Biol. 156: 1089-1098.

O'Reilly LA, Ekert P, Harvey N, Marsden V, Cullen L, Vaux DL, Hacker G, Magnusson C, Pakusch M, Cecconi F, Strasser A, Huang DCS, Kumar S (2002) Caspase-2 is not required for thymocyte or neuronal apoptosis even though cleavage of caspase-2 is mediated by Apaf-1 and caspase-9. Cell Death Diff. 9: 832-841.

Cakouros D, Daish T, Martin D, Baehrecke EH, Kumar S (2002) Ecdysone-induced expression of the caspase Dronc during hormone dependent programmed cell death in Drosophila is regulated by Broad-Complex. J. Cell Biol. 157: 985-995.

Baliga BC, Kumar S (2002) Role of Bcl-2 family of proteins in malignancy. Hematol. Oncology. 20: 63-74.

Richardson H, Kumar S (2002) Death to flies: Drosophila as a model system to study programmed cell death. J. Immunol. Meth. 265: 21-38.

Konstas A-A, Shearwin-Whyatt LM, Fotia A, Degger B, Riccardi D, Cook DI, Korbmacher C, Kumar S (2002) Regulation of the epithelial sodium channel by N4WBP5A, a novel Nedd4/Nedd4-2-interacting protein. J. Biol. Chem. 277: 29406-29416.

Read SH, Baliga BB, Ekert P, Vaux DL, Kumar S (2002) A novel Apaf-1-independent putative caspase-2 activation complex. J. Cell Biol. 159: 739-745.

Kumar S, Vaux DL (2002) A Cinderella caspase takes center stage. Science 297: 1290-1291.

Selected earlier publications

Dinudom A, Harvey KF, Komwatana P, Jolliffe CN, Young JA, *Cook DI, *Kumar S (2001) The roles of the carboxyl termini of a-, b- and g-subunits of epithelial Na+ channels (ENaC) in regulating ENaC and in mediating its inhibition by cytosolic Na+. J. Biol. Chem. 276: 13744-13749. (*equal senior authors)

Harvey KF, Dinudom A, Cook DI, Kumar S (2001) The Nedd4-like protein KIAA0439 is a potential regulator of the epithelial sodium channel. J. Biol. Chem. 276: 8597-8601.

Doumanis J, Quinn L, Richardson H, Kumar S (2001) STRICA, a novel Drosophila caspase with an unusual serine/threonine-rich prodomain, interacts with DIAP1 and DIAP2. Cell Death Diff. 8: 387-394.

Harvey NL, Daish T, Mills K, Dorstyn L, Quinn LM, Read SH, Richardson H, Kumar S (2001) Characterization of the Drosophila caspase, DAMM. J. Biol. Chem. 276: 25342-25350.

Shearwin-Whyatt LM, Harvey NL, Kumar S (2000) Subcellular localization and CARD-dependent oligomerization of the death adaptor RAIDD. Cell Death Diff. 7: 155-165.

Kumar S, Doumanis J (2000) The fly caspases. Cell Death Differ. 7: 1039-1044.

Colussi PA, Quinn LM, Huang DCS, Coombe M, Read SH, Richardson H, Kumar S (2000) Debcl, a pro-apoptotic Bcl-2 homologue, is a component of the Drosophila cell death machinery. J. Cell Biol. 148: 703-714.

Jolliffe CN, Harvey KF, Haines BP, Parasivam G, Kumar S (2000) Identification of multiple proteins expressed in murine embryos as binding partners for the WW domains of the ubiquitin-protein ligase Nedd4. Biochem. J. 351: 557-565.

Quinn LM, Dorstyn L, Mills K, Colussi PA, Chen P, Coombe M, Abrams J, *Richardson H, *Kumar S (2000) An essential role for the caspase Dronc in developmentally programmed cell death in Drosophila. J. Biol Chem.275: 40416-40424. (*equal senior authors)

Kumar S, Colussi PA (1999) Prodomains-adaptors-oligomerisation: the pursuit of caspase activation in apoptosis. Trends Biochem. Sci. 24: 1-4.

Harvey KF, Dinudom A, Komwatana P, Jolliffe CN, Day ML, Parasivam G, Cook DI, Kumar S (1999) All three WW domains of murine Nedd4 are involved in the regulation of epithelial sodium channels by intracellular Na+. J. Biol. Chem. 274: 12525-12530.

Dorstyn L, Colussi PA, Quinn LM, Richardson H, Kumar S (1999) DRONC, an ecdysone-inducible Drosophila caspase. Proc. Natl. Acad. Sci. USA 96: 4307-4312.

Ishibashi H, Dinudom A, Harvey KF, Kumar S, Young JA, Cook DI (1999) Na+-H+ exchange in salivary secretary cells is controlled by an intracellular Na+ receptor. Proc. Natl. Acad. Sci. USA 96: 9949-9953.

Morrione A, Plant P, Valentinis B, Staub O, Kumar S, Rotin D and Baserga R (1999) mGrb10 interacts with Nedd4. J. Biol. Chem. 274: 24094-24099.

Dorstyn L, Read SH, Quinn LM, Richardson H, Kumar S (1999) DECAY, a novel Drosophila caspase related to mammalian caspase-3 and caspase-7. J. Biol. Chem. 274: 30778-30783.

Harvey KF, Kumar S (1999) Nedd4-like proteins: an emerging family of ubiquitin-protein ligases implicated in diverse cellular functions. Trends Cell Biol. 9: 166-169.

Kumar S (1999) Mechanisms of caspase activation in cell death. Cell Death Diff. 6: 1060-1066.

Dinudom A, Harvey KF, Komwatana P, Young JA, Kumar S, Cook DI (1998) Nedd4 mediates control of an epithelial Na+ channel in salivary duct cells by cytosolic Na+. Proc. Natl. Acad. Sci. USA 95: 7169-7173.

Butt AJ, Harvey NL, Parasivam G, Kumar S (1998) Dimerization and autoprocessing of the Nedd2 (caspase-2) precursor requires both the prodomain and the carboxyl terminal regions. J. Biol. Chem. 273: 6763-6768.

Harvey KF, Harvey NL, Michael JM, Parasivam G, Waterhouse N, Alnemri ES, Watters D, Kumar S (1998) Caspase-mediated cleavage of the ubiquitin-protein ligase Nedd4 during apoptosis. J. Biol. Chem. 273: 13524-13530.

Bird CH, Sutton VR, Sun J, Hirst CE, Novak A, Kumar S, Trapani JA, Bird PI (1998) Selective regulation of apoptosis: the cytotoxic lymphocyte serpin PI-9 protects against granzyme B-mediated apoptosis without perturbing the Fas cell death pathway. Mol. Cell. Biol. 18: 6387-6398.

Colussi PA, Harvey NL, Kumar S (1998) Prodomain-dependent nuclear localization of the caspase-2 (Nedd2) precursor: A novel function for a caspase prodomain. J. Biol. Chem. 273: 24535-24542.

Waterhouse NJ, Finucane D, Green DR, Elce JS, Kumar S, Alnemri E, Litwack G, Lavin MF, Watters D (1998) Calpain activation is upstream of caspases in irradiation-induced apoptosis. Cell Death Diff. 5: 1051-1061.

Colussi PA, Harvey NL, Shearwin-Whyatt LM, Kumar S (1998) Conversion of procaspse-3 to an autoactivating caspase by fusion to the caspase-2 prodomain. J. Biol. Chem. 273: 26566-26570.

Kumar S, Kinoshita M, Dorstyn L, Noda M (1997) Origin, expression and possible functions of the two alternatively spliced forms of the mouse Nedd2 mRNA. Cell Death Diff. 4: 378-387.

Kumar S, Harvey KF, Kinoshita M, Copeland NG, Noda M, Jenkins NA (1997) cDNA cloning, expression analysis and mapping of the mouse Nedd4 gene. Genomics 40: 435-443.

Kinoshita M, Kumar S, Mizoguchi A, Ide C, Kinoshita A, Haraguchi T, Hiraoka Y, Noda M (1997) Nedd5, a mammalian septin, is a novel cytoskeletal component interacting with actin-based structures. Genes Dev. 11: 1535-1547.

Harvey NL, Butt A, Kumar S (1997) Functional activation of Nedd2/ICH-1 (caspase-2) is an early process in apoptosis. J. Biol. Chem. 272: 13134-13139.

Kumar S, Lavin MF (1996) The ICE family of cysteine proteases as effectors of cell death. Cell Death Diff. 3: 255-267.

Song Q, Lees-Miller SP, Kumar S, Zhang N, Chan DW, Smith GCM, Jackson SP, Alnemri ES, Litwack G, Khanna KK, Lavin MF (1996) DNA-dependent protein kinase catalytic subunit: A target for an ICE-like protease in apoptosis. EMBO J.  15: 3238-3246.

Song Q, Burrows S, Lees-Miller S, Smith G, Jackson S, Kumar S, Trapani JA, Alnemri E, Litwack G, Lu H, Moss D, Lavin MF (1996) ICE-like protease cleaves DNA-dependent protein kinase in cytotoxic T-cell killing. J. Exp. Med. 184: 619-626.

Waterhouse N, Kumar S, Song Q, Strike P, Sparrow L, Dreyfuss G, Alnemri ES, Litwack G, Lavin M, Watters D (1996) Heteronuclear ribonucleoproteins C1 and C2, components of the spliceosome, are specific targets of interleukin-1beta-converting enzyme-like proteases in apoptosis. J. Biol. Chem. 271: 29335-29341.

Kumar S (1995) ICE-like proteases in apoptosis. Trends Biochem. Sci. 20: 198-202.

Kumar S, Matsuzaki T, Yoshida Y, Noda M (1994) Molecular cloning and biological activity of a novel developmentally regulated gene encoding a protein with transducin beta-like structure. J. Biol. Chem. 269: 11318-11326.

Kumar S, Kinoshita M, Noda M, Copeland NG, Jenkins NA (1994) Induction of apoptosis by mouse Nedd2 gene, which encodes a protein similar to the product of the Caenorhabditis elegans cell death gene ced-3 and the mammalian IL-1beta-converting enzyme. Genes Dev. 8: 1613-1626.

Kumar S, Yoshida Y, Noda M (1993) Cloning of a cDNA which encodes a novel ubiquitin-like protein. Biochem. Biophys. Res. Commun. 195: 393-399.

Kumar S, Tomooka Y, Noda M (1992) Identification of a set of genes with developmentally down-regulated expression in the mouse brain. Biochem. Biophys. Res. Commun. 185: 1155-1161.

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